Adelaide defeated Brisbane by nine points, with an ugly moment costing the Lions at Springfield Central Stadium.
Brisbane's Lily-Rose Williamson was penalised for wrapping her arms around the neck of Adelaide's Teah Charlton inside the forward 50, allowing for Charlton to slot a third-quarter goal.
Elsewhere, Gold Coast, Richmond and West Coast all enjoyed victories.
Brisbane's wait to ignite its AFLW season will continue, as an ugly moment contributed to its nine-point loss to Adelaide.
Last year's grand finalists entered Sunday's clash at Springfield Central Stadium to find form after suffering their third loss in four games with a five-point defeat to West Coast last Saturday.
The midfielder scored from the ensuing free kick and the Lions didn't recover until late in the final term as the visitors secured a 5.9 to 4.6 victory.
The Lions were left stunned as the Crows held them goalless in the first quarter while kicking two majors of their own.
Crows forward India Rasheed grabbed a major, but teenager Asher Fearn-Wannan and midfielder Catherine Svarc kicked consolation goals.
Gold Coast's band of youngsters have strengthened their grip on a third AFLW finals berth, beating Essendon by 41 points at Windy Hill.
Their 7.9 to 1.4 win over Essendon on Sunday was their third consecutive victory and moved them into fifth spot, just four points behind second-placed defending champions North Melbourne.
Fresh off a 15-point win over St Kilda last Sunday, the Tigers raced out of the gates and led by 16 points at quarter-time in Canberra.